1. Specifications

Globalsil NH₂ (amino) columns use spherical high-purity silica with propylaminosilane bonded as the stationary phase, and can be used in both normal-phase and reversed-phase modes.

In normal-phase mode, typical mobile phases include n-hexane/ethyl acetate or isopropanol/ethanol, primarily for separating polar compounds such as aromatic amine derivatives, lipids, steroids, and chlorinated pesticides. In reversed-phase mode, common mobile phases are acetonitrile/water or methanol/water, used for separating monosaccharides, disaccharides, polysaccharides, and carbohydrates. It can also act as a weak anion-exchange phase to separate phenols, organic carboxylic acids, and nucleotides.

Note: When switching the same amino column between normal-phase and reversed-phase modes, use isopropanol as an intermediate solvent to avoid incompatibility between normal-phase and reversed-phase mobile phases.

Various specifications available for selection:

Note: Other particle sizes of 3, 5, 15, 50 μm are available. Please contact us for inquiring and ordering!

2. Applications

Acarbose

Column: GlobalSIL SP-120-5-APS (250 mm × 4.6 mm, 5 μm)

Mobile Phase: Phosphate buffer (Dissolve 600 mg potassium dihydrogen phosphate and 279 mg anhydrous disodium hydrogen phosphate in water, and dilute to 1000 mL) - acetonitrile (29:71, v/v)

Flow Rate: 2.0 mL/min; Column Temperature: 35 °C

Detector: UV detector at 210 nm

Honey

Column: GlobalSIL SP-120-5-APS (250 mm × 4.6 mm, 5 μm)

Mobile Phase: Acetonitrile-Water (75:25, v/v)

Flow Rate: 1.0 mL/min

Detector: RID