UNISIL C18-BP chromatography packing material is particularly suitable for environments with aqueous mobile phases containing low organic solvent content. Under such high-aqueous chromatographic conditions, the brush-like C18 chains of conventional stationary phases produced using traditional bonding technologies will collapse, leading to poor peak shapes. However, UNISIL C18-BP can maintain excellent peak shapes in these mobile phases. This advantage greatly improves the selectivity of the column in aqueous mobile phases, which is fully reflected when analyzing hydrophilic and polar substances. Typical applications include the separation of biomolecules and metabolites, such as oligosaccharides, amino acids, small peptides, nucleotides, and organic acids.

UNISIL C18-BP stationary phase is fully endcapped and possesses similar selectivity to conventional C18 stationary phases when separating hydrophobic compounds in reversed-phase mode.

UNISIL C18-BP stationary phase features a stable baseline and high sensitivity in neutral and ion-free environments, making it highly suitable for various hyphenated techniques, such as LC-MS.

Mixed Vitamins

Column: UNISIL 120-5-C18-BP, 150 mm × 4.6 mm, 5 μm

Mobile Phase: A: 20 mM ammonium dihydrogen phosphate aqueous solution; B: Acetonitrile; Gradient elution

Flow Rate: 1.0 mL/min; Column Temperature: 35°C

Detection Wavelength: 280 nm