The dual separation mechanism of capillary electrophoresis can simultaneously separate ionized and neutral compounds. Due to the use of electroosmosis driving, there is no backpressure issue present in liquid chromatography, allowing for the use of 1.5μm ultra-small particle fillers, resulting in higher column efficiency, better peak shapes, and larger peak capacities. Longer capillary columns can be used compared to liquid chromatography, achieving better separation effects. The electrically driven flow's plug-like flow pattern improves the flow pattern and dispersion of sample molecules inside the capillary column, enabling significantly higher column efficiencies than those achieved in liquid chromatography.
We provide various types and sizes of fillers for users of capillary electrophoresis columns, such as C8, C18, phenyl, cyanopropyl, silica, ion exchange, chiral fillers, etc., with particle sizes ranging from 1.5μm to 5μm. We also offer different inner diameters and lengths of capillary tubes for selection. Inner diameters include 50μm, 75μm, 100μm, 150μm, and 320μm, while the lengths range from 20cm to 50cm.
Capillary column stationary phase and its applications:
C8-C8H17 Non-polar Reverse Phase: Suitable for moderately polar to nonpolar compounds, solutes that dissolve in water like small peptides, proteins, steroids (steroids), nucleosides, nucleotides, highly polar synthetic drugs.
C18-C18H37 Phenyl-C6H5 Non-Polar Reverse Phase: Suitable for nonpolar to moderately polar compounds such as fatty acids, triglycerides, polycyclic aromatic hydrocarbons, esters (neopentyl glycol esters), fat-soluble vitamins, steroids (steroids), PTH derivatives of amino acids.
Amino-(CH2)3NH2 Polar Normal Phase: Suitable for polar compounds like phenylurea analogs, fats, steroids, chlorinated pesticides. Reverse phase can separate monosaccharides, disaccharides, and polysaccharides; Anion exchange can separate phenols, organic carboxylic acids, nucleotides.
Cyano-(CH2)3CN Polar Normal Phase: Similar to silica gel adsorbent for hydrogen bonding acceptors. Suitable for analyzing polar compounds with lower retention values than silica columns in reverse phase, providing different selectivity compared to C8, C18, phenyl columns.
Strong Anion Exchange (CH2)3C6H8SO3H - Strong Anion Exchange Organic Base / Strong Anion Exchange Organic Acid: Suitable for separating nucleotides and nucleosides using silica-OH polar normal phase or reverse phase. Also separates chiral compounds with enantiomers on a chiral stationary phase.
Other details:
- Transportation:By air if not specified.
