New solution and selection

The qCE system brings a breakthrough change for the traditional CE！

  

      A quantitative capillary electrophoresis was developed by utilizing a rotary type of nano-volume injector, an auto-sampler, and a thermostat with cooling capacity. The nano-injector with an accurate internal loop provides a new solution for the problem of accurate quantification in CE. The accuracy and precision were greatly improved compared with conventional CE.

 

Introduction of core modules

 

Auto sampler

All of the modules of the quantitative CE system were autom atically controlled by a computer program.

 

Nano-injector

1. The injection volume accuracy was guaranteed by the carefully designed nano-injector with an accurate internal loop 

2. Applies to all types of samples and have no discrimination effects

3. Continuous on-line analysis can be performed and have no need to interrupt the process of electrical balance

4. Have no cross-contamination between the samples and the buffers

5. Valve injection is simple, convenient and safe

 

Electrical decoupler

1. Allowed electrical current to flow between the buffer and capillary but without permeating the buffer and sample to 

pass through the membrane on the capillary 

2. Realizing the technology of powering on capillary 

3. Isolating the injector from the electrical field

 

Thermostat

1. Temperature control medium: liquid 

2. Temperature range: 4~40℃

 

	Items	Description
Overall performance	Qualitative reproducibility(%)	＜0.8
	Quantitative reproducibility(%)	＜1.0
Solvent delivery system	Range (mL/min)	0.001-10.000
	Precision (% RSD)	≤0.1
	Accuracy (%)	±2
Autosampler	Injection precision	＜1.0
	Injection accuracy	＜2.0
UV/Vis	Range (nm)	190-700
	‍‍Precision (nm)	≤1
	Repeatability (nm)	≤0.1
	‍‍‍‍Band Width (nm)	8
	Baseline noise (AU)	±0.25*10-5
	Baselinedrift (AU/h)	＜1.0*10-4
	LOD (g/mL)	≤1.0*10-6
‍‍‍‍‍‍High voltage power supply	Voltage range (kV)	0-±30
	Current range (μA)	0-100
	Voltage stability	≤1.5% (in 3 min)
Working environment requirement ‍	Workingtemperature	15-30
	Storage temperature	0-40
	Relative humidity	＜90%
	Power supply	220 V ± 10%
	Power frequency (Hz)	50 ± 0.5

 

Determination of six synthetic adulterants in weight-loss products

 Capillary: 50 μm i.d., 360 μm o.d.

  Effective length/ Overall length: 40 cm/60 cm

  Buffer:  20 mM NaH₂PO₄/H₃PO₄(pH2.0)

  Voltage: 15 kV

  Pressurized flow by syringe pump: 0.6 μL/min. 

  Split ratio (no electric field) : 10:1

  Temperature: 15℃

  Wavelength： 195 nm

  Injection volume: 10 nL

  Samples: 1. pseudoephedrine      2. norepinephrine              3. fenfluramine              

                  4. clenbuterol                5. sibutramine                    6. Amfebutamone

 

 

 

Determination of five preservatives in cosmetics

Capillary: 50 μm i.d., 360 μm o.d.

Effective length/ Overall length: 40 cm/60 cm

Buffer: 15 mM sodium borate +100 mM SDS (pH 9.30) 

Voltage: 20 kV

Temperature: 15℃

Wavelength: 254 nm

Injection volume: 10 nL

Pressurized flow by syringe pump: 1 μL/min

Split ratio (no electric field): 13:1

Samples: 1. MP     2. benzoic acid     3. EP     4. PP        5. BP

 

 

 

 

The baseline separation of six nucleosides

Capillary: 50 μm i.d., 360 μm o.d.

Effective length/ Overall length: 40 cm/60 cm

Voltage: 15 kV

Wavelength: 254 nm

Injection volume: 10 nL

Temperature: 12 ℃

Buffer: 30 mM sodium borate (pH 9.40)

Pressurized flow by syringe pump: 1 μL/min.

Split ratio (no electric field) : 13:1

Samples: (a)cytosine            (b) 5-fluoro-2'–deoxyuridine                   (c) adenosine      

               (d) uracil                (e) uridine                                                  (f) creatinine