Principle diagram (combining high-performance liquid chromatography and capillary electrophoresis)

High-performance micro-flow electrochromatography is a new type of micromolecular separation analysis technology developed by combining the advantages of two modern separation techniques: high-performance liquid chromatography and capillary electrophoresis. By applying direct-current high-pressure electric fields at both ends of the capillary column or coated column, the sample's retention behavior in the capillary column is affected simultaneously by electrophoresis and its distribution coefficient between the mobile phase and stationary phase. Under the action of dual separation mechanisms, the pressure-assisted capillary electrochromatography has significantly enhanced its ability to separate micro-molecules, improving the analysis capacity, and representing a trend in high-efficiency micromolecular separation in analytical science. It is particularly suitable for studying complex biological and chemical systems.

Main features of eHPLC:

• Three high, one fast: High column efficiency, high resolution, high selectivity, and rapid separation.
• Significantly saves solvent and sample consumption.
• Easily combined with MS, μLIF, ECD, μELSD for hyphenation.

Solvent delivery system

Flow rate range: 1 μl/min - 10 ml/min

Flow accuracy: ±1%

Flow precision: 0.06% RSD

Pressure resistance: 1.0 - 40 MPa

UV-Visible detector on capillary column

Light source: Deuterium lamp

Wavelength range: 190 - 700 nm

Spectral bandwidth: 8 nm

Wavelength accuracy: ±1 nm

Wavelength repeatability: ±0.1 nm

Baseline drift: 1×10^-4 AU/h

Baseline noise: ±0.25×10^-5 AU

Sensitivity: 0.0001 - 2.56 AUFS

Light source lifetime: 2000 hours (D2 deuterium lamp)

High-pressure power supply

High-efficiency separation of four polycyclic aromatic hydrocarbons (PAHs)

Amazing column efficiency, approximately 600,000 theoretical plates per meter!

Capillary column: EP100-25-1.5-ODS

Mobile phase: 70% CH3CN/30% 4mM sodiumtetraborate (pH 9.1)

Voltage: 20 kV

Detector: LIF, excitation wavelength: 257 nm, emission wavelength: 400 nm

Samples: a) Fluorene, b) Benzofluorene, c) Benzo(a)fluorene, d) Benzo(c)phenanthrene

High-resolution separation of 14 explosives

Separation effect and speed far surpass traditional liquid chromatography methods!

Capillary column: EP-75-15-1.5-ODS

Mobile phase: 15% CH3OH/85% 10mM MES (pH 8.5)

Voltage: 12 kV

Detector: UV, 254 nm

Electropherograms of human plasma from patients with pancreatic cancer and healthy individuals (eHPLC-UV)

Capillary column: 30 cm (effective) ×150 μm i.d., ODS, 5 μm;

Mobile phase A: 5% MeOH (0.05%TFA)

Mobile phase B: 95% MeOH/ACN(70:30)(0.05%TFA)

Gradient: 0-5min, 10%B; 5-10min, 10-25%B; 10-45min, 25-100%B; 45-80min, 100%B;

Voltage: -4 kV

Flow rate: 0.06 mL/min;

UV detection: 214 nm

Sample volume: 52 nL