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Pressurized Capillary Electrochromatography
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Product name:eHPLC/pCEC
Product model:TriSep®3000
Product overview

eHPLC/pCEC is designed to combine the advantages of HPLC and CE. 

It offers a new solution to separate and analyze complex compounds and the cousumption of samples and solvent is just 1/1000 of conventional HPLC.


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  • The mobile phase is driven to pass through the stationary phase by both pressure and EOF and the sample is therefore separated due to the chromatographic partition and electrophoretic mobility. Therefore, ionic and neutral compounds can be separated at the same time

    As the exist of electroosmotic flow, there is no worry about the back pressure .Therefore, ultra small particles can be used to get higher efficiency, better peak shape and lager peak capacity. And longer column can be used for better separation. The elcetroosmotic flow helps improve the sample pattern and molecule diffusion in the column, contributing to a far better column efficiency than HPLC.

    Advantages:

      1. Separation of charged and non-charged components at the same time

      2. Ultra high efficiency with the use of very small particles

      3. Low consumption of solvent and sample

      4. Fast speed of separation

      5. pCEC,nano-LC and CE,all in one

      6. Ready to couple with μUV, μLIF, μECD, μELSD,  μMS


  • solvent delivery system

    ItemDescription
    Range1.0 μL/min-10 mL/min
    Accuracy±1.0%
    Precision0.1%
    Pressure range1.0-40 MPa

    UV/vis detector 

    ItemDescription
    Light sourcedeuterium lamp
    Wavelength range190~700 nm
    Spectral bandwidth8.0 nm
    Wavelength accuracy±1.0 nm
    Wavelength reproducibility±0.1 nm
    Baseline Drift2.0×10-4 AU/h
    Baseline noise5.0×10-6 AU

    High voltage power

    ItemDescription
    Voltage range0~±30 kV
    Current range0~±300  μA


  • eHPLC/pCEC can be widely used in proteomics, metabonomics, drug analysis, food safety, environmental protection, university and research institute.


    Application 1:  Rapid separtion of 16 PAHs by eHPLC/pCEC- LIF

        Capilliary column: 100 µm i.d. × 20 cm, 1.5 µm C18 

        Voltage: 29 kV, Sample: 20 nL

        Mobile phase: 65% (v/v) CH3 CN/35% 2 mM TRIS

        Detector: LIF, Ex.: 257 nm, Em.: 400 nm


    Application 2:  Separtion of FITC-labeled Aliphatic Amines by eHPLC/pCEC- LIF 

     


      Column: 100 µm i.d. × 20 cm, 3.0µm C18  

      Mobile phase:

      A:10%(v/v) ACN + 10%40 mM PBS+H20

      B: 65%(v/v) ACN + 10%40 mM PBS+H20

      Pressure: 1500 psi

      Power voltage:5 kV

      Detector: LIF ,  Ex.: 473 nm,  Em.: 520 nm






    Application 3:  Separtion of  Chinese traditional medicines by eHPLC/pCEC- ELSD



    Column: 150 µm i.d. × 15 cm, 3 µm C18          

    Flow rate: 1.02 µL/min

    Mobile phase A: CH3CN􀀃

    Mobile phase B: 10 mol/L methane acid-triethylamine 

    Voltage: 10 kV

    Sample: 20 nL

    Detector: ELSD

    Gas flow rate: 0.4 L/min

    Evaporative temperature: 40℃






    Application 4:  Analysis of Microsystin LR and RR by eHPLC-MS




    Column: 100 µm i.d. × 25 cm, 1.8 µm C18 

    Flow rate: 0.1 mL/min

    Pressure: 10 Mpa

    Sample: 0.01 mg/mL Mc LR and RR

    Mobile phase: ACN/H O (V/V=70/30) + 0.1% FA

     Detector: MSD

    Column temperature: 25℃ 

    Injection volume: 1.0 µL

    Voltage: 10 kV



    Application 5:  Analysis of BSA digest by eHPLC-UV



    Column: 100 µm i.d. × 25 cm, 1.9 µm C18

    Mobile phase A: ACN + 0.1% FA 

    Mobile phase B: H2O + 0.1% FA

    Gradient elution

    Flow rate: 0.1 mL/min 

    Column temperature: 25℃ 

    Detection wavelength: 214 nm

    Pressure: 17 MPa

    Injection volume: 3.5 nL

    Voltage: 15 kV